Functional Assay:Article Title: Prolonging parathyroid hormone analog action in vitro and in vivo through peptide lipidation
Article Snippet: .. Functional assays were performed in stably transfected cell lines derived from HEK-293 cells (ATCC Cat. # CRL-1573) and expressing the glosensor cAMP reporter (plasmid p22f, Promega) along with either the human PTH1R (GP-2.3 cells), pHL2-hPTH1R, in which pHluorin2, a pH-sensitive GFP variant, is inserted into the E2 region of the hPTH1R’s ECD (GPG-10 cells), PTH1R-delNt yfp , in which the receptor’s N-terminal extracellular domain (ECD) is replaced by yellow fluorescent protein (GD-5y cells), or β-Arrestin2 yfp (GBR-24 cells). .. Assays were also performed in SGS-72 cells, which are derived from the human osteoblastic cell line Saos-2 (ATCC Cat. # HTB-85) and stably express glosensor.
Stable Transfection:Article Title: Prolonging parathyroid hormone analog action in vitro and in vivo through peptide lipidation
Article Snippet: .. Functional assays were performed in stably transfected cell lines derived from HEK-293 cells (ATCC Cat. # CRL-1573) and expressing the glosensor cAMP reporter (plasmid p22f, Promega) along with either the human PTH1R (GP-2.3 cells), pHL2-hPTH1R, in which pHluorin2, a pH-sensitive GFP variant, is inserted into the E2 region of the hPTH1R’s ECD (GPG-10 cells), PTH1R-delNt yfp , in which the receptor’s N-terminal extracellular domain (ECD) is replaced by yellow fluorescent protein (GD-5y cells), or β-Arrestin2 yfp (GBR-24 cells). .. Assays were also performed in SGS-72 cells, which are derived from the human osteoblastic cell line Saos-2 (ATCC Cat. # HTB-85) and stably express glosensor.
Transfection:Article Title: Prolonging parathyroid hormone analog action in vitro and in vivo through peptide lipidation
Article Snippet: .. Functional assays were performed in stably transfected cell lines derived from HEK-293 cells (ATCC Cat. # CRL-1573) and expressing the glosensor cAMP reporter (plasmid p22f, Promega) along with either the human PTH1R (GP-2.3 cells), pHL2-hPTH1R, in which pHluorin2, a pH-sensitive GFP variant, is inserted into the E2 region of the hPTH1R’s ECD (GPG-10 cells), PTH1R-delNt yfp , in which the receptor’s N-terminal extracellular domain (ECD) is replaced by yellow fluorescent protein (GD-5y cells), or β-Arrestin2 yfp (GBR-24 cells). .. Assays were also performed in SGS-72 cells, which are derived from the human osteoblastic cell line Saos-2 (ATCC Cat. # HTB-85) and stably express glosensor.
Derivative Assay:Article Title: Prolonging parathyroid hormone analog action in vitro and in vivo through peptide lipidation
Article Snippet: .. Functional assays were performed in stably transfected cell lines derived from HEK-293 cells (ATCC Cat. # CRL-1573) and expressing the glosensor cAMP reporter (plasmid p22f, Promega) along with either the human PTH1R (GP-2.3 cells), pHL2-hPTH1R, in which pHluorin2, a pH-sensitive GFP variant, is inserted into the E2 region of the hPTH1R’s ECD (GPG-10 cells), PTH1R-delNt yfp , in which the receptor’s N-terminal extracellular domain (ECD) is replaced by yellow fluorescent protein (GD-5y cells), or β-Arrestin2 yfp (GBR-24 cells). .. Assays were also performed in SGS-72 cells, which are derived from the human osteoblastic cell line Saos-2 (ATCC Cat. # HTB-85) and stably express glosensor.
Expressing:Article Title: Prolonging parathyroid hormone analog action in vitro and in vivo through peptide lipidation
Article Snippet: .. Functional assays were performed in stably transfected cell lines derived from HEK-293 cells (ATCC Cat. # CRL-1573) and expressing the glosensor cAMP reporter (plasmid p22f, Promega) along with either the human PTH1R (GP-2.3 cells), pHL2-hPTH1R, in which pHluorin2, a pH-sensitive GFP variant, is inserted into the E2 region of the hPTH1R’s ECD (GPG-10 cells), PTH1R-delNt yfp , in which the receptor’s N-terminal extracellular domain (ECD) is replaced by yellow fluorescent protein (GD-5y cells), or β-Arrestin2 yfp (GBR-24 cells). .. Assays were also performed in SGS-72 cells, which are derived from the human osteoblastic cell line Saos-2 (ATCC Cat. # HTB-85) and stably express glosensor.
Article Title: Prolonging parathyroid hormone analog action in vitro and in vivo through peptide lipidation.
Article Snippet: .. # CRL-1573) and expressing the glosensor cAMP reporter (plasmid p22f, Promega) along with either the human PTH1R (GP-2.3 cells), pHL2-hPTH1R, in which pHluorin2, a pH-sensitive GFP variant, is inserted into the E2 region of the hPTH1R’s ECD (GPG-10 cells), PTH1R-delNtyfp, in which the receptor’s N-terminal extracellular domain (ECD) is replaced by yellow fluorescent protein (GD-5y cells), or β-Arrestin2yfp (GBR-24 cells). .. Assays were also performed in SGS-72 cells, which are derived from the humanosteoblastic cell line Saos-2 (ATCC Cat. # HTB-85) and stably express glosensor.
Plasmid Preparation:Article Title: Prolonging parathyroid hormone analog action in vitro and in vivo through peptide lipidation
Article Snippet: .. Functional assays were performed in stably transfected cell lines derived from HEK-293 cells (ATCC Cat. # CRL-1573) and expressing the glosensor cAMP reporter (plasmid p22f, Promega) along with either the human PTH1R (GP-2.3 cells), pHL2-hPTH1R, in which pHluorin2, a pH-sensitive GFP variant, is inserted into the E2 region of the hPTH1R’s ECD (GPG-10 cells), PTH1R-delNt yfp , in which the receptor’s N-terminal extracellular domain (ECD) is replaced by yellow fluorescent protein (GD-5y cells), or β-Arrestin2 yfp (GBR-24 cells). .. Assays were also performed in SGS-72 cells, which are derived from the human osteoblastic cell line Saos-2 (ATCC Cat. # HTB-85) and stably express glosensor.
Article Title: Prolonging parathyroid hormone analog action in vitro and in vivo through peptide lipidation.
Article Snippet: .. # CRL-1573) and expressing the glosensor cAMP reporter (plasmid p22f, Promega) along with either the human PTH1R (GP-2.3 cells), pHL2-hPTH1R, in which pHluorin2, a pH-sensitive GFP variant, is inserted into the E2 region of the hPTH1R’s ECD (GPG-10 cells), PTH1R-delNtyfp, in which the receptor’s N-terminal extracellular domain (ECD) is replaced by yellow fluorescent protein (GD-5y cells), or β-Arrestin2yfp (GBR-24 cells). .. Assays were also performed in SGS-72 cells, which are derived from the humanosteoblastic cell line Saos-2 (ATCC Cat. # HTB-85) and stably express glosensor.
Variant Assay:Article Title: Prolonging parathyroid hormone analog action in vitro and in vivo through peptide lipidation
Article Snippet: .. Functional assays were performed in stably transfected cell lines derived from HEK-293 cells (ATCC Cat. # CRL-1573) and expressing the glosensor cAMP reporter (plasmid p22f, Promega) along with either the human PTH1R (GP-2.3 cells), pHL2-hPTH1R, in which pHluorin2, a pH-sensitive GFP variant, is inserted into the E2 region of the hPTH1R’s ECD (GPG-10 cells), PTH1R-delNt yfp , in which the receptor’s N-terminal extracellular domain (ECD) is replaced by yellow fluorescent protein (GD-5y cells), or β-Arrestin2 yfp (GBR-24 cells). .. Assays were also performed in SGS-72 cells, which are derived from the human osteoblastic cell line Saos-2 (ATCC Cat. # HTB-85) and stably express glosensor.
Article Title: Prolonging parathyroid hormone analog action in vitro and in vivo through peptide lipidation.
Article Snippet: .. # CRL-1573) and expressing the glosensor cAMP reporter (plasmid p22f, Promega) along with either the human PTH1R (GP-2.3 cells), pHL2-hPTH1R, in which pHluorin2, a pH-sensitive GFP variant, is inserted into the E2 region of the hPTH1R’s ECD (GPG-10 cells), PTH1R-delNtyfp, in which the receptor’s N-terminal extracellular domain (ECD) is replaced by yellow fluorescent protein (GD-5y cells), or β-Arrestin2yfp (GBR-24 cells). .. Assays were also performed in SGS-72 cells, which are derived from the humanosteoblastic cell line Saos-2 (ATCC Cat. # HTB-85) and stably express glosensor.
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